Assembly breakdown and you may genome pointers away from K. michiganensis BD177

Assembly breakdown and you may genome pointers away from K. michiganensis BD177

Performance

1.3 Gbp) were processed to remove SMRT bell adapters, short and low-quality reads (< 80% accuracy) using SMRT Analysis version 2.3 ( A total of 155,828 filtered reads (average length, 8.9 Kb) were used for de novo assembly using Celera Assembler , with self-correction of the PacBio reads. A total of 1091 Mb paired-end Illumina reads screened from a 500-bp genomic library were mapped using SOAP to the bacterial plasmid database for tracing the presence of any plasmid and filling the gaps. The de novo assembly resulted in five contigs, representing the K. michiganensis BD177 complete genome of 6,812,698 bp with GC content % in a single chromosome and four plasmids. The annotated genome contains 6714 genes, 25rRNA genes, and 86 tRNA genes (Table 1).

16S rRNA phylogenetic investigation and you may phenotypic away from isolated strain BD177

Limit parsimony oriented and you can maximum probability phylogenetic trees utilizing the series you to encodes towards the 16S rRNA gene showed that the closest species to help you BD177 are K. michiganensis and you may K. oxytica (More file step one: Fig. S1, Extra document 2: Dining table S1). Evolutionary divergence length from 16S rRNA gene series between BD177 and you will K. michiganensis E718 are lowest worthy of (BD177 compared to K. michiganensis E718: 0.0079) one of all the sequences. Biochemical screening had been did into the BD177 because of the API20E to confirm their condition regarding K. oxytoca and K. michiganensis. Biochemical functions regarding K. oxytoca ATCC13182(T)(=NCTC13727) and K. michiganensis W14(T)(= DSM2544) was in fact obtained from BacDive Webservices pared with K. oxytoca ATCC13182(T) and you may K. michiganensis W14(T), BD177 is the same which have W14 on the ?-galactosidase enzyme self-confident and you will urease bad, same which have ATCC13182(T) from inside the arginine dihydrolase positive.